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The Long chain fatty acyl-CoA synthetase enzyme is a member of the ligase family that activates the breakdown of complex fatty acids. Long chain fatty acyl-CoA synthetase plays a crucial role in intermediary metabolism by catalyzing the formation of fatty acyl-CoA by a two-step process proceeding through an adenylated intermediate.

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Antigen retrieval is a technique in which the masking of epitope is reversed and epitope antibody binding is restored. Ph value of retrieval solution is depending on which solution you are using.